Lightnovo
Application notes
Application note: miniRaman Spectrometer for Analysis of Honey and Syrups
The Lightnovo miniRaman spectrometer showcases its advanced capabilities in the analysis of honey and syrups, providing a compact and efficient solution for differentiating high-sucrose products like sugar syrups from low-sucrose or natural sweeteners such as honey. This application note demonstrates how miniRaman is a reliable tool for ensuring the authenticity and quality of liquid sweet food products.
Samples Analyzed with the miniRaman spectrometer:
Samples Analyzed with the miniRaman spectrometer:
- Natural Bee Honey: A late summer honey sourced from Denmark, with a sucrose content of ≤5%.
- Maple Syrup: A commercially available product made with natural sugar substitutes like sorbitol, erythritol, and steviol glycosides.
- Sweet Syrup: Another commercial syrup using the same natural sweeteners as maple syrup.
- Sugar Syrup: A homemade sucrose solution consisting of water and white sugar.
Using the miniRaman spectrometer, these samples were analyzed with minimal preparation, demonstrating its ability to differentiate between complex compositions and detect potential adulteration. This makes the miniRaman an essential tool for quality control and product authentication in the food industry.This makes the miniRaman an essential tool for quality control and product authentication in the food industry.
Application Note: Raman Spectroscopy of Over-the-Counter Pharmaceuticals and Supplements
This Application Note highlights the power of Raman spectroscopy in analyzing and identifying over-the-counter medicines and health supplements, with the innovative miniRaman spectrometer.
Samples Analyzed
Samples Analyzed
- Whey Protein
- Creatine Monohydrate
- Vitamin C
- Multivitamins
- Ibuprofen
- Paracetamol
Lightnovo’s miniRaman spectrometer, equipped with a high-precision contact probe and powered by Miraspec software, enables direct Raman spectroscopy analysis through plastic packaging. This innovative approach ensures rapid, non-invasive testing while delivering high-quality, reproducible results. The system achieved exceptional accuracy, with hit quality indices (HQI) above 97% for most tested samples.Key benefits of Lightnovo’s solution include:
- Non-destructive analysis suitable for pharmaceutical and supplement testing.
- Ease of use for both laboratory and field applications.
- High sensitivity for reliable compound identification.
Application Note: miniRaman Microscope for In-Vitro Bacteria Identification
Lightnovo’s miniaturized Raman microscope supports bacterial diagnostics by providing rapid, label-free bacteria identification and antimicrobial susceptibility testing (AST). This compact and accessible system delivers high accuracy and speed for advanced bacterial analysis.
Samples Analyzed:
Samples Analyzed:
- Bacteria were cultured on agar plates and transferred directly to sterilized CaF₂ slides for consistent preparation.
- The study targeted key bacterial strains, including E. coli, MRSE, MSSE, and S. lugdunensis.
- Using Raman maps and machine learning, the system achieved 98.6% accuracy in bacterial classification with a reduced exposure time of 0.2 seconds per spectrum.
Lightnovo’s compact Raman technology offers precision, speed, and accessibility, setting a new standard for bacterial diagnostics. Discover how this innovative solution can enhance your research and diagnostic capabilities.
Explore its potential today.
Explore its potential today.
Explore its potential today.
Application Note: Microplastics Detection in Seawater Using 2D Raman Mapping
Lightnovo’s compact Raman microscopes provide an innovative and efficient approach to analyzing microplastics (MPs) in seawater. This advanced technology enables direct, on-site detection of MPs on filters, eliminating the need for additional sample preparation and ensuring fast, accurate results.
Samples Analyzed:
Samples Analyzed:
- Seawater Collection: A 10-liter sample from the Mediterranean Sea was filtered using a silicon membrane, and the residue was analyzed directly on the filter.
- Raman Specifications: The system used a 532 nm laser to scan a 10×10 mm² field with a 2 µm spot size, capturing high-resolution spectral data across 600×600 points.
Lightnovo’s Raman microscopy offers a practical and effective solution for real-time microplastics detection. Its compact design and reliable performance make it suitable for both field monitoring and laboratory use, supporting environmental research and pollution control efforts.
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Application note: In-vivo Skin Measurements with miniRaman Spectrometer
The Lightnovo miniRaman spectrometer is a compact and efficient solution for non-invasive in-vivo skin measurements. Designed for high-precision analysis, it provides detailed insights into the molecular composition of the skin, from the surface to depths of up to 150 µm.
Samples and Results:
Samples and Results:
- Skin Areas Analyzed: Finger, hand, and cheek at depths of 10–20 µm.
- Raman Spectra Observed: Clear differentiation in water content across areas using CH (2800–3000 cm⁻¹) and OH (3100–3500 cm⁻¹) bands.
- Application Example: Penetration of anti-sun cream (SPF30) was successfully measured.
The miniRaman spectrometer, paired with a high-NA immersion probe, achieves a signal-to-noise ratio (SNR) of 500:1 within a 1-second exposure time, demonstrating its capability for efficient, reproducible skin analysis.
This powerful miniRaman technology is ideal for skin diagnostics and studying the penetration and permeation of topical formulation
This powerful miniRaman technology is ideal for skin diagnostics and studying the penetration and permeation of topical formulation
Application Note: Quantification of Anti-Cancer Drug via SERS Mapping
miniRaman microscope for Monitoring Methotrexate
Therapeutic Drug Monitoring (TDM) is essential for drugs like methotrexate (MTX) that have a narrow therapeutic window. Lightnovo’s miniaturized Raman microscope provides an innovative solution for SERS mapping to enable accurate MTX quantification with improved limits of detection.
Key Highlights:
- Instrumentation: Measurements were conducted using Lightnovo’s miniaturized Raman microscope with SERS substrates.
- Enhanced Sensitivity: Achieved a Limit of Detection (LoD) of 3 μM and Limit of Quantification (LoQ) of 20 μM, surpassing previously reported results.
- SERS Mapping: MTX concentrations ranging from 0 to 75 μM were analyzed with a total measurement time of 15 minutes per chip, demonstrating the system’s speed and reliability.
Conclusion:
Lightnovo’s miniRaman microscope delivers precise and efficient SERS mapping for therapeutic drug monitoring. This innovative technology is a valuable tool for researchers and clinicians, enabling better patient outcomes through accurate drug monitoring.
Application Note: Quantification of Toxic Methanol in Vodka
Miniaturized Raman Spectroscopy for the detection of toxic Methanol in Vodka
Methanol contamination in low-quality or counterfeit alcoholic beverages poses serious health risks. Lightnovo’s miniaturized Raman spectrometers provide a rapid, reliable, and non-invasive method to identify and quantify methanol directly through glass bottles.
Key Highlights:
Key Highlights:
- Instrumentation: Measurements were conducted using Lightnovo’s miniRaman spectrometer equipped with a 785 nm laser.
- Accurate Quantification: Achieved a limit of detection (LoD) as low as 0.07% and a limit of quantification (LoQ) of 0.25%, exceeding the sensitivity of conventional Raman systems.
- Non-Destructive Testing: Methanol concentrations in water-ethanol solutions were measured without opening the bottles, ensuring fast and effective analysis.
Conclusion:
Lightnovo’s Raman technology enables precise methanol detection, ensuring safety and compliance with regulations. This compact solution supports industries in combating counterfeit alcohol and protecting public health.
Lightnovo’s Raman technology enables precise methanol detection, ensuring safety and compliance with regulations. This compact solution supports industries in combating counterfeit alcohol and protecting public health.
Application Note: Miniaturized Raman Microscope for Nutrient and Metabolite Quantification in Fermentation
Lightnovo’s miniaturized Raman microscope is transforming the monitoring of microbial fermentation processes. Designed to measure nutrients, metabolites, and by-products in real-time, this compact technology ensures high precision and efficiency, making it an ideal solution for process control in industries like pharmaceuticals and biotechnology.
Samples Analyzed:
Samples Analyzed:
- Nutrients and Metabolites: The system quantified critical components such as pHCA, glucose, MgSO₄, and Na₂HPO₄ during E. coli fermentation.
- Correlated Analysis: Results from Raman spectroscopy showed strong alignment with HPLC data, confirming its accuracy and reliability for fermentation monitoring.
Lightnovo’s miniaturized Raman spectrometer offers a high-performance, cost-effective solution for monitoring and quantifying nutrients and metabolites. Its compact design and real-time capabilities make it a valuable tool for optimizing fermentation processes and ensuring consistent product quality.
Application Note: miniRaman Spectroscopy for Analysis of Christmas Tree Needles
Discover how Lightnovo’s miniRaman spectrometers provide precise and efficient analysis of Christmas tree needles. By leveraging advanced Raman spectroscopy, this study demonstrates how shifting excitation wavelengths reduces fluorescence interference, enhancing spectral clarity.
Key Highlights:
Key Highlights:
- Instrumentation: Analysis conducted using Lightnovo miniRaman 785 nm and miniRaman 820 nm spectrometers
- Benefits: The advanced capabilities of the miniRaman spectrometers allow for clear, accurate data collection even in complex organic samples.
Lightnovo’s miniRaman technology showcases its versatility and precision, offering solutions for diverse applications, including detailed organic material analysis. This innovative approach ensures reliable results across various use cases.
Explore its potential today.
Application Note: Raman Spectroscopy of Pumpkin Skin
MiniRaman Spectroscopyfor Analyzing Pumpkin Skin
Discover the unique chemical composition of pumpkin skin with Lightnovo’s miniRaman spectrometer. This study showcases how Raman spectroscopy reveals the presence of carotenoids responsible for the vibrant orange color of pumpkins.
Key Highlights:
- Instrumentation: Measurements were conducted using the Lightnovo miniRaman Power 785 nm spectrometer with a middle-distance probe (15 mm) and settings of 91 mW power.
- Advanced Corrections: Baseline corrections were applied to eliminate fluorescence background, revealing clear Raman signals.
- Chemical Insights: Raman spectra identified carotenoids such as beta-carotene, lutein, and zeaxanthin, compounds that contribute to the characteristic orange color of pumpkin skin.
Conclusions:
Lightnovo’s miniRaman 785 nm spectrometer is a reliable tool for analyzing natural compounds in organic materials like pumpkin skin. This compact device offers precise spectral analysis, making it ideal for both research and educational purposes.